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- Caspase 3 Antibodies
Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
View all (50) Caspase 3 antibodies
Cite Caspase 3 Polyclonal Antibody
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Caspase 3 Antibody (PA5-77887) in WB
Western blot was performed using Anti-Caspase 3 Polyclonal Antibody (Product # PA5-77887) and a 32 kDa band corresponding to Caspase-3 was observed across U-87 MG, NTERA-2 cl.D1, HeLa and Jurkat. Whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), NTERA-2 cl.D1 (Lane 2), HeLa (Lane 3), Jurkat (Lane 4) and Jurkat treated with 1 µM of Staurosporine for 3 hours (Lane 5) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB2300... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Product Details
PA5-77887
Applications
Tested Dilution
Publications
Western Blot(WB)
1:500-1:10,000
View 2 publications2 publications
Immunohistochemistry (IHC)
-
View 8 publications8 publications
Immunohistochemistry (Paraffin) (IHC (P))
1:100-1:1,000
View 1 publication1 publication
Immunohistochemistry (Frozen)(IHC (F))
1:100-1:1,000
-
Immunocytochemistry(ICC/IF)
1:100-1:1,000
-
Immunoprecipitation(IP)
1:100-1:500
-
Product Specifications
Species Reactivity
Human,Mouse,Rat
Published species
Mouse,Rat
Host/Isotype
Rabbit/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Caspase 3. The exact sequence is proprietary.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.15 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2735574
Product Specific Information
Positive Control: HeLa, HeLa (30 µM Cisplatin treatment for 24 hr), PC-12 (30uM cisplatin treatment for 24h), PC-12, Rat2, C8D30, NIH-3T3, Raw264.7, C2C12, Jurkat, Raji, NCI-H929, K562, THP-1, HL-60
Predicted Reactivity: Dog (89%), Cat (87%), Pig (88%), Rabbit (86%), Chimpanzee (99%), Bovine (84%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases:Apopain; Apopain precursor; CASP-3; Caspase 3 apoptosis related cysteine protease (ICE-like cysteine protease); caspase 3, apoptosis related cysteine protease; Caspase 3, apoptosis related cysteine protease (ICE-like cysteine protease); caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; Caspase-3; Caspase3; CPP-32; CPP32beta; Cysteine protease CPP32; ICE3; IRP; LICE; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; SREBP cleavage activity 1; yama protein
Gene Aliases:A830040C14Rik; AC-3; Apopain; CASP-3; CASP3; Caspase-3; CC3; CPP-32; CPP32; CPP32B; Lice; mldy; SCA-1; Yama
UniProt ID:(Human) P42574, (Mouse) P70677, (Rat) P55213
Entrez Gene ID:(Human) 836, (Mouse) 12367, (Rat) 25402
Molecular Function: 
protease
Function(s)
response to hypoxiaB cell homeostasisapoptotic DNA fragmentationproteolysisapoptotic processactivation of cysteine-type endopeptidase activity involved in apoptotic processcellular component disassembly involved in execution phase of apoptosiscellular response to DNA damage stimulusheart developmentsensory perception of soundlearning or memoryactivation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome cresponse to UVresponse to glucoseresponse to X-rayregulation of macroautophagyprotein processinghippocampus developmentneuron differentiationkeratinocyte differentiationerythrocyte differentiationplatelet formationnegative regulation of B cell proliferationresponse to cobalt ionresponse to estradiolresponse to lipopolysaccharideglial cell apoptotic processresponse to tumor necrosis factorresponse to nicotinehippo signalingwound healingresponse to drugresponse to hydrogen peroxideT cell homeostasisnegative regulation of apoptotic processresponse to amino acidpositive regulation of neuron apoptotic processcell fate commitmentnegative regulation of cyclin-dependent protein serine/threonine kinase activitynegative regulation of activated T cell proliferationresponse to antibioticneurotrophin TRK receptor signaling pathwayresponse to glucocorticoidneuron apoptotic processcellular response to organic cyclic compoundapoptotic signaling pathwayextrinsic apoptotic signaling pathway in absence of ligandexecution phase of apoptosisresponse to woundingintracellular signal transductionpositive regulation of apoptotic processpositive regulation of catalytic activitynegative regulation of cell cyclecellular response to organic substancerelease of cytochrome c from mitochondriaextrinsic apoptotic signaling pathway via death domain receptorsintrinsic apoptotic signaling pathway in response to oxidative stressresponse to organic substanceresponse to metal ionresponse to organic cyclic compoundregulation of apoptotic DNA fragmentation
Process(es)
protease bindingaspartic-type endopeptidase activitycysteine-type endopeptidase activitycyclin-dependent protein serine/threonine kinase inhibitor activitydeath receptor bindingprotein bindingpeptidase activitycysteine-type endopeptidase activator activity involved in apoptotic processphospholipase A2 activator activityprotein complex bindingcysteine-type endopeptidase activity involved in apoptotic processcysteine-type endopeptidase activity involved in execution phase of apoptosiscysteine-type peptidase activityhydrolase activity
It has to be done as per old AB suggested Products section.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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